Back to Biology

Subject Content - GCSE Biology

Topic B1: Cell level systems

Topic B1 covers the cell level systems. Cells are the fundamental units of living organisms. They contain sub-cellular structures essential for the functioning of the cell as a whole. Microscopy is used to examine cells and sub-cellular structures.

B1.1 Cell structures

Summary, underlying knowledge, misconceptions, and tiering for B1.1 Cell structures.

  • Summary: Cells are the fundamental units of living organisms. Cells contain many sub-cellular structures that are essential for the functioning of the cell as a whole. Microscopy is used to examine cells and sub-cellular structures.
  • Underlying knowledge and understanding: Learners should be familiar with cells as the fundamental unit of living organisms, and with the use of light microscopes to view cells. They should also be familiar with some sub-cellular structures, and the similarities and differences between plant and animal cells.
  • Common misconceptions: Learners commonly have difficulty understanding the concept of a cell as a 3D structure, so this should be addressed during the teaching of this topic.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B1.1 Cell structures - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM1.1idemonstrate an understanding of number, size and scale and the quantitative relationship between unitsM2a and M2h
BM1.1iiuse estimations and explain when they should be usedM1d
BM1.1iiicalculate with numbers written in standard formM1b

B1.1 Cell structures - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B1.1a describe how light microscopes and staining can be used to view cellslenses, stage, lamp, use of slides and cover slips, and the use of stains to view colourless specimens or to highlight different structures/tissues and calculation of magnificationM1b, M1d, M2a, M2hWS1.2c, WS1.4c, WS1.4d, WS1.4e, WS2a, WS2b, WS2c, WS2dInvestigation of a range of cells using pictures, light micrographs and diagrams. Measure the size and magnification of the cells. (PAG B1, PAG B7)

Preparation of cheek cell slides. (PAG B7)

Preparation of onion epidermis cells slides. (PAG B7)

Use of light microscopes to view plant and animal cells. (PAG B7)
B1.1b explain how the main sub-cellular structures of eukaryotic cells (plants and animals) and prokaryotic cells are related to their functionsnucleus, genetic material, chromosomes, plasmids, mitochondria (contain enzymes for cellular respiration), chloroplasts (contain chlorophyll) and cell membranes (contain receptor molecules, provides a selective barrier to molecules), ribosomes (site of protein synthesis)WS1.4a, WS2a, WS2b, WS2c, WS2dProduction of 3D model plant and animal cells to illustrate their differences.

Investigation of cytoplasmic streaming in Elodea spp. (PAG B6, PAG B7)
B1.1c explain how electron microscopy has increased our understanding of sub-cellular structuresincreased resolution in a transmission electron microscopeM1bWS1.1a, WS1.4c, WS1.4dComparison of a range of cells using pictures from light and electron micrographs.

Comparison of the visible structures visible on light and electron micrographs.

B1.2 What happens in cells (and what do cells need)?

Life processes depend on biological molecules whose structure is related to their function. Inside every cell is genetic material and this is used as a code to make proteins. Enzymes are important proteins in biology.

B1.2 Context & Overview

  • Underlying knowledge and understanding: Learners should have a simple understanding of the double helix model of DNA. Learners should be familiar with the idea of enzymes as biological catalysts.
  • Common misconceptions: Learners commonly hold the misconception that DNA is made of protein or sugar. Learners also think that all enzymes have an optimum temperature of 37°C (human body temperature). The range of optimum temperatures of enzymes should be introduced through the teaching of this topic and further addressed when considering homeostatic mechanisms for controlling temperature.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B1.2 Cell systems - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM1.2icarry out rate calculations for chemical reactionsM1a and M1c
BM1.2iiunderstand and use simple compound measures such as the rate of a reactionM1a and M1c

B1.2 Cell systems - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B1.2a describe DNA as a polymerWS1.4aProduction of 3D models of DNA to illustrate its structure.
B1.2b describe DNA as being made up of two strands forming a double helix
B1.2c describe that DNA is made from four different nucleotides; each nucleotide consisting of a common sugar and phosphate group with one of four different bases attached to the sugarthe pairs of complementary bases (A-T and G-C)WS1.4a, WS2a, WS2b, WS2c, WS2dProduction of 3D models of DNA to illustrate its structure.

Investigation of DNA extraction from a living organism (e.g. kiwi, leek, onion, wheat germ). (PAG B2)
B1.2d recall a simple description of protein synthesisthe unzipping of the DNA molecule around the gene, copying to mRNA in nucleus (transcription), (translation) of the nucleotide sequence in the cytoplasm, tRNA as the carrier of amino acidsComparison of transcription and translation to a non-lending library.

Use of kinaesthetic activities to demonstrate transcription and translation.
B1.2e explain simply how the structure of DNA affects the proteins made in protein synthesistriplet code and its use to determine amino acid order in a protein
B1.2f describe experiments that can be used to investigate enzymatic reactionsM1a, M1c, M2gWS1.1h, WS1.2b, WS1.2c, WS1.2e, WS1.3a, WS1.3b, WS1.3c, WS1.3d, WS1.3e, WS1.3f, WS1.3g, WS2a, WS2b, WS2c, WS2dInvestigations of enzyme activity, including numerical analysis of data and graphical representation of results. (PAG B2, PAG B4, PAG B6)
B1.2g explain the mechanism of enzyme actionthe role of enzymes in metabolism, the role of the active site, enzyme specificity (lock and key hypothesis) and factors affecting the rate of enzyme controlled reactions (pH, temperature, substrate and enzyme concentration)M1a, M1c, M3d, M4bWS2a, WS2b, WS2c, WS2dInvestigation into the effect of amylase on a baby rice paste. (PAG B2, PAG B4, PAG B6)

Investigation of enzyme controlled reactions. (PAG B2, PAG B4, PAG B6)

Work out rate equations using simple algebraic equations. (PAG B4)

B1.3 Respiration

Metabolic processes such as respiration are controlled by enzymes. Organic compounds are used as fuels in cellular respiration to allow the other chemical reactions necessary for life.

B1.3 Context & Overview

  • Underlying knowledge and understanding: Learners should also have some underpinning knowledge of respiration. This should include that respiration involves the breakdown of organic molecules to enable all the other chemical processes necessary for life. Learners should be able to recall the word equation for respiration.
  • Common misconceptions: Learners commonly hold the misconception that ventilation is respiration. They can also get confused between the terms breakup and breakdown.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B1.3 Respiration - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B1.3a describe cellular respiration as a universal chemical process, continuously occurring that supplies ATP in all living cellsWS1.2a
B1.3b describe cellular respiration as an exothermic reactionWS1.2bDemonstration of an exothermic reaction (e.g. heat pack).
B1.3c compare the processes of aerobic respiration and anaerobic respirationin plants/fungi and animals the different conditions, substrates, products and relative yields of ATPWS2a, WS2b, WS2c, WS2dResearch into whether plants respire. (PAG B2, PAG B4, PAG B5, PAG B6)

Investigation of fermentation in fungi. (PAG B2, PAG B4, PAG B5, PAG B6)

Investigation of respiration in yeast using alginate beads to immobilise the fungus. (PAG B2, PAG B4, PAG B5, PAG B6)
B1.3d explain the importance of sugars in the synthesis and breakdown of carbohydratesuse of the terms monomer and polymerDemonstration of the synthesis and breakdown of biological molecules (e.g. using Lego bricks). Qualitative testing of biological molecules PAG B2
B1.3e explain the importance of amino acids in the synthesis and breakdown of proteinsuse of the terms monomer and polymerQualitative testing of biological molecules PAG B2
B1.3f explain the importance of fatty acids and glycerol in the synthesis and breakdown of lipidsQualitative testing of biological molecules PAG B2

B1.4 Photosynthesis

Life processes depend on photosynthesis. Green plants and algae trap light from the Sun to fix carbon dioxide with hydrogen from water making organic compounds.

B1.4 Context & Overview

  • Underlying knowledge and understanding: Learners should also have some underpinning knowledge of photosynthesis. They should have an understanding that plants make carbohydrates in their leaves by photosynthesis, and be able to recall the word equation for photosynthesis.
  • Common misconceptions: Learners often think that plants do not respire.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B1.4 Photosynthesis - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM1.4iunderstand and use simple compound measures such as the rate of a reactionM1a and M1c
BM1.4iitranslate information between graphical and numerical formM4a
BM1.4iiiplot and draw appropriate graphs, selecting appropriate scales and axesM4a and M4c
BM1.4ivextract and interpret information from graphs, charts and tablesM2c and M4a
BM1.4vunderstand and use inverse proportion - the inverse square law and light intensity in the context of factors affecting photosynthesisM1c

B1.4 Photosynthesis - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B1.4a describe photosynthetic organisms as the main producers of food and therefore biomass for life on EarthUse of concept cartoons to start discussions about photosynthesis.
B1.4b describe the process of photosynthesisreactants and products, two-stage process, location of the reaction (in the chloroplasts)WS2a, WS2b, WS2c, WS2dInvestigation of photosynthesis e.g. the Priestley experiment using Cabomba to collect oxygen or the Ingenhousz experiment to show mass gain. (PAG B4, PAG B5, PAG B6)
B1.4c describe photosynthesis as an endothermic reactionWS1.3b, WS1.3c, WS1.3eDemonstration of an endothermic reaction (e.g. icepack).
B1.4d describe experiments to investigate photosynthesisWS2a, WS2b, WS2c, WS2dExperiments to show the consequences of light exclusion on photosynthesising plants (e.g. testing geraniums for starch). (PAG B4, PAG B5, PAG B6)
B1.4e explain the effect of temperature, light intensity and carbon dioxide concentration on the rate of photosynthesisM1a, M1c, M4a, M4b, M4c, M2gWS2a, WS2b, WS2c, WS2dInvestigation of photosynthesis in algae using alginate beads to immobilize the algae. (PAG B4, PAG B5, PAG B6)
B1.4f explain the interaction of temperature, light intensity and carbon dioxide concentration in limiting the rate of photosynthesisusing graphs depicting the effects of the limiting factorsM1d, M2c, M4a, M1cWS1.2b, WS1.2c, WS1.2e, WS1.3a, WS1.3b, WS1.3c, WS1.3d, WS1.3f, WS1.3g, WS1.4e, WS2c, WS2d

Topic B2: Scaling up

Topic B2 covers the mechanisms of transport and scaling up within biological systems.

B2.1 Supplying the cell

  • Summary: Cells transport many substances across their membranes by diffusion, osmosis and active transport. Stem cells are found in both plants and animals. These stem cells can divide, differentiate and become specialised to form tissues, organs and organ systems.
  • Underlying knowledge and understanding: Learners should be familiar with the role of diffusion in the movement of materials in and between cells.
  • Common misconceptions: Learners commonly show some confusion regarding surface area: volume ratio, particularly how larger animals have a smaller surface area: volume ratio. They also show some confusion as to stem cells: where they are found and their roles. Care should be taken to give clear definitions when covering this content.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B2.1 Supplying the cell - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM2.1iuse percentiles and calculate percentage gain and loss of massM1c

B2.1 Supplying the cell - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B2.1a explain how substances are transported into and out of cells through diffusion, osmosis and active transportexamples of substances moved, direction of movement, concentration gradients and use of the term water potential (no mathematical use of water potential required)M1c, M1dWS2a, WS2b, WS2c, WS2dObservation of osmosis in plant cells using a light microscope.

Investigation of 'creaming yeast' to show osmosis. (PAG B6, PAG B8)

Investigation into changes in mass of vegetable chips when placed in sucrose/salt concentrations of varying concentrations. (PAG B6, PAG B8)
B2.1b describe the process of mitosis in growth, including the cell cyclethe stages of the cell cycle as cell growth, DNA replication, more cell growth, movement of chromosomesWS2a, WS2b, WS2c, WS2dModelling of mitosis using everyday objects e.g. shoes, socks etc.

Observation of mitosis in stained root tip cells. (PAG B1, PAG B6, PAG B7)
B2.1c explain the importance of cell differentiationthe production of specialised cells allowing organisms to become more efficient and examples of specialised cellsWS2a, WS2b, WS2c, WS2dExamination of a range of specialised cells using a light microscope. (PAG B1)
B2.1d recall that stem cells are present in embryonic and adult animals, and meristems in plantsDemonstration of cloning using cauliflower. (PAG B6, PAG B7)
B2.1e describe the functions of stem cells in embryonic and adult animals, and meristems in plantsdivision to produce a range of different cell types for development, growth and repairWS1.1e, WS1.1f, WS1.1h
B2.1f describe the difference between embryonic and adult stem cells in animalsResearch into the different types of stem cells.

B2.2 The challenges of size

When organisms become multicellular, the need arises for highly adapted structures including gaseous exchange surfaces and transport systems, enabling living processes to be performed effectively.

B2.2 Context & Overview

  • Underlying knowledge and understanding: Learners should be familiar with the role of diffusion in the movement of materials in and between cells. They should also be familiar with the human gaseous exchange system.
  • Common misconceptions: Learners have a view that the slow flow of blood in capillaries is due to the narrow diameter, when in fact it is a function of the total cross-sectional area of the capillaries (1000 times greater than the aorta). When explaining the importance of the slow flow of blood in allowing time for exchange by diffusion, this misunderstanding should be considered.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B2.2 The challenges of size - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM2.2icalculate surface area : volume ratiosM1c
BM2.2iiuse simple compound measures such as rateM1a and M1c
BM2.2iiicarry out rate calculationsM1a and M1c
BM2.2ivplot, draw and interpret appropriate graphsM4a, M4b, M4c and M4d

B2.2 The challenges of size - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B2.2a explain the need for exchange surfaces and a transport system in multicellular organisms in terms of surface area : volume ratiocalculation of surface area, volume and surface area : volume ratio, and reference to diffusion distancesM1cWS1.4d, WS1.4e, WS1.4f, WS2a, WS2b, WS2c, WS2dInvestigating surface area : volume ratio using hydrochloric acid and gelatine cubes stained with phenolphthalein or other suitable pH indicator. (PAG B8)
B2.2b describe some of the substances transported into and out of a range of organisms in terms of the requirements of those organismsoxygen, carbon dioxide, water, dissolved food molecules, mineral ions and urea
B2.2c describe the human circulatory systemthe relationship with the gaseous exchange system, the need for a double circulatory system and the arrangement of vesselsModelling of the human circulatory system.
B2.2d explain how the structure of the heart and the blood vessels are adapted to their functionsthe structure of the mammalian heart with reference to the cardiac muscle, the names of the valves, chambers, and blood vessels into and out of the heart, the structure of the blood vessels with reference to thickness of walls, diameter of lumen, presence of valvesWS2a, WS2b, WS2c, WS2dInvestigating heart structure by dissection.

Investigation of a blood smear using a light microscope. (PAG B1)

Modelling of blood using sweets to represent the components.
B2.2e explain how red blood cells and plasma are adapted to their transport functions in the bloodWS2a, WS2b, WS2c, WS2dExamine the gross structure of blood vessels using a light microscope. (PAG B1)

Investigating the elasticity of different blood vessels using hanging masses.
B2.2f explain how water and mineral ions are taken up by plants, relating the structure of the root hair cells to their functionWS2a, WS2b, WS2c, WS2dExamination of root hair cells using a light microscope. (PAG B1)

Demonstration of the effectiveness of transpiration by trying to suck water from a bottle using a 10m straw. (PAG B8)

Investigation of the position of the xylem/phloem in root, stem and leaf tissues using a light microscope. (PAG B1)

Interpretation of experimental evidence of the movement of dissolved food materials in a plant. (PAG B1, PAG B8)

Examining the position of the phloem in root, stem and leaf tissues using a light microscope. (PAG B1)
B2.2g describe the processes of transpiration and translocationthe structure and function of the stomataWS2a, WS2b, WS2c, WS2dMeasurement of plant stomatal density by taking an impression of the leaf using clear nail varnish or spray-on plaster. (PAG B1, PAG B6, PAG B8)
B2.2h explain how the structure of the xylem and phloem are adapted to their functions in the plant
B2.2i explain the effect of a variety of environmental factors on the rate of water uptake by a plantlight intensity, air movement, and temperatureM1a, M1c, M1dWS2a, WS2b, WS2c, WS2dInterpreting experimental evidence of investigations into environmental factors that affect water uptake. (PAG B6, PAG B8)
B2.2j describe how a simple potometer can be used to investigate factors that affect the rate of water uptakecalculation of rate and percentage gain/loss of massM1a, M1c, M1d, M2g, M3d, M4a, M4b, M4c, M4dWS1.2b, WS1.2c, WS1.2e, WS1.3a, WS1.3b, WS1.3c, WS1.3d, WS1.3e, WS1.3f, WS1.3g, WS2a, WS2b, WS2c, WS2dInvestigation of transpiration rates from a plant cutting. (PAG B6, PAG B8)

Work out the rate of transpiration in volume of water/time. (PAG B6, PAG B8)

Topic B3: Organism level systems

Topic B3 covers coordination, control and the organism level systems.

B3.1 Coordination and control – the nervous system

  • Summary: The human nervous system is an important part of how the body communicates with itself and also receives information from its surroundings.
  • Underlying knowledge and understanding: Learners should have a concept of the hierarchical organism of multicellular organisms from cells to tissues to organs to systems to organisms.
  • Common misconceptions: Learners commonly think that their eyes see objects 'directly', like a camera, but the reality is that the image formed by the brain is based on the eyes and brains interpretation of the light that comes into the eye i.e. different people will perceive the same object or image differently. Young learners also have the misconception that some sort of 'force' comes out of the eye, enabling it to see.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B3.1 Nervous system - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM3.1iextract and interpret data from graphs, charts and tablesM2c

B3.1 Nervous system - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B3.1a describe the structure of the nervous systemCentral Nervous System, sensory, motor and relay neurones, sensory receptors, synapse and effectors, details of the structure of sensory and motor neurones requiredProduction of 3D models of neurones to illustrate their structure.
B3.1b explain how the components of the nervous system can produce a coordinated responseit goes to all parts of the body, has many links, has different sensory receptors and is able to coordinate responsesDemonstration (by video) of someone trying to do everyday tasks whilst being given mild electric shocks (e.g. BBC Brainiac).
B3.1c explain how the structure of a reflex arc is related to its functionM1d, WS2a, WS2b, WS2c, WS2dDemonstration of reaction time by getting a learner to catch a falling £5 note.

Research into reflexes. (PAG B6)

Investigating of reaction times by ruler drop. (PAG B6)
B3.1d explain how the main structures of the eye are related to their functionscornea, iris, pupil, lens, retina, optic nerve, ciliary body, suspensory ligamentsDemonstration of the inversion of an image through a beaker full of water.

Demonstration of the features of the human eye.

Investigation of eye structure by dissection. (PAG B1)
B3.1e describe common defects of the eye and explain how some of these problems may be overcomecolour blindness, short-sightedness and long-sightednessWS2a, WS2b, WS2c, WS2dMeasurement of focal length in a variety of situations. (PAG B6)

Research into eye defects, their diagnosis and treatment.
B3.1f describe the structure and function of the braincerebrum, cerebellum, medulla, hypothalamus, pituitary
B3.1g explain some of the difficulties of investigating brain functionthe difficulty in obtaining and interpreting case studies and the consideration of ethical issuesDiscussion of problems associated with brain research including the difficulty in getting research subjects.
B3.1h explain some of the limitations in treating damage and disease in the brain and other parts of the nervous systemlimited ability to repair nervous tissue, irreversible damage to the surrounding tissues, difficulties with accessing parts of the nervous systemWS1.1e, WS1.1f, WS1.1hResearch into a study of brain injury.

B3.2 Coordination and control – the endocrine system

Hormones are chemical messengers. In animals, hormones are transported around the body in the blood and affect target tissues and organs. Hormones have a variety of roles in the human body, including controlling reproduction. Plant hormones are chemicals that regulate plant growth and development. They can be used in agriculture to control the rate of growth.

B3.2 Context & Overview

  • Underlying knowledge and understanding: Learner should be aware of a number of hormones including adrenaline and the male and female sex hormones.
  • Common misconceptions: With regards to the menstrual cycle, research has shown that learners have problems relating the time of conception to the condition of the lining of the uterus.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B3.2 Endocrine system - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM3.2iextract and interpret data from graphs, charts and tablesM2c
BM3.2iitranslate information between numerical and graphical formsM4a

B3.2 Endocrine system - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B3.2a describe the principles of hormonal coordination and control by the human endocrine systemuse of chemical messengers, transport in blood, endocrine glands and receptors
B3.2b explain the roles of thyroxine and adrenaline in the bodythyroxine as an example of a negative feedback system
B3.2c describe the role of hormones in human reproduction including the control of the menstrual cycleoestrogen, progesterone, FSH and testosteroneWS1.3b, WS1.3e
B3.2d explain the interactions of FSH, LH, oestrogen and progesterone in the control of the menstrual cycleM2c, M4a, M2gAnalysis of relative hormones levels from raw data and graphically.
B3.2e explain the use of hormones in contraception and evaluate hormonal and non-hormonal methods of contraceptionrelative effectiveness of the different forms of contraceptionM2c, M4aWS1.1d, WS1.1e, WS1.1fDiscussion into the various methods of contraception and their effective/ethical use.
B3.2f explain the use of hormones in modern reproductive technologies to treat infertilityWS1.1d, WS1.1e, WS1.1f, WS1.1hResearch into Xenopus laevis pregnancy testing to detect hCG by the stimulation of oogenesis.

Research into hormonal treatments for infertility.
B3.2g explain how plant hormones are important in the control and coordination of plant growth and development, with reference to the role of auxins in phototropisms and gravitropismsunequal distribution of auxinWS2a, WS2b, WS2c, WS2dInvestigation of the effects of phototropism using seedlings. (PAG B6)
B3.2h describe some of the variety of effects of plant hormones, relating to auxins, gibberellins and ethenecontrolling growth, controlling germination, fruit ripening, flower opening and shedding of leavesWS2a, WS2b, WS2c, WS2dInvestigation/research into the question 'does one bad banana spoil the fruit bowl?' (PAG B2, PAG B6)
B3.2i describe some of the different ways in which people use plant hormones to control plant growthselective herbicides, root cuttings, seedless fruit (parthenocarpic fruit development), altering dormancy

B3.3 Maintaining internal environments

Homeostasis is crucial to the regulation of internal environments and enables organisms to adapt to change, both internally and externally. Internal temperature, blood sugar levels and osmotic balance are regulated by a number of organs and systems working together.

B3.3 Context & Overview

  • Underlying knowledge and understanding: Learners will build on the knowledge and understanding gained in section 3.1 about coordination and control when considering the topics in this section.
  • Common misconceptions: Learners often confuse type 1 and type 2 diabetes, and the effective treatments for each. The effect of ADH on the permeability of the kidney tubules is often confused.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B3.3 Maintaining internal environments - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM3.3iextract and interpret data from graphs, charts and tablesM2c

B3.3 Maintaining internal environments - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B3.3a explain the importance of maintaining a constant internal environment in response to internal and external changeallowing metabolic reactions to proceed at appropriate ratesWS1.4aResearch into hypothermia.
B3.3b describe the function of the skin in the control of body temperaturedetection of external temperature, sweating, shivering, change to blood flow in terms of vasoconstriction and vasodilationWS2a, WS2b, WS2c, WS2dDemonstration of the cooling effect of sweating using alcohol based surgical wipes. (PAG B6)

Investigation into heat loss by using microwaved plasticine shapes/model 'animals' by using a UV heat camera/thermometers. (PAG B6)
B3.3c explain how insulin controls blood sugar levels in the bodyM2g
B3.3d explain how glucagon interacts with insulin to control blood sugar levels in the bodyM2cWS2a, WS2b, WS2c, WS2dInvestigations into the glucose content of artificial urine to diagnose diabetes, using e.g. Clinistix. (PAG B6)
B3.3e compare type 1 and type 2 diabetes and explain how they can be treated
B3.3f explain the effect on cells of osmotic changes in body fluidshigher, lower or equal water potentials leading to lysis or shrinking (no mathematical use of water potentials required)WS2a, WS2b, WS2c, WS2dDemonstration of the different water potentials on different cells. (PAG B6, PAG B8)
B3.3g describe the function of the kidneys in maintaining the water balance of the bodyvarying the amount and concentration of urine and hence water excretedWS1.3b, WS2a, WS2b, WS2c, WS2dInvestigation of the structure of the kidney by dissection and the application of H2O2 to visualise the nephrons. (PAG B6, PAG B8)

Investigations into the glucose content of artificial urine to diagnose diabetes, using e.g. Clinistix. (PAG B6)
B3.3h describe the gross structure of the kidney and the structure of the kidney tubuleBowman's capsule, proximal convoluted tubule, loop of Henle and collecting duct
B3.3i describe the effect of ADH on the permeability of the kidney tubulesamount of water reabsorbed and negative feedbackWS2a, WS2b, WS2c, WS2dInvestigation of the different sections of a nephron and the composition of the filtrate from each area. (PAG B2, PAG B6, PAG B8)
B3.3j explain the response of the body to different temperature and osmotic challengeschallenges to include high sweating and dehydration, excess water intake, high salt intake responses to include mechanism of kidney function, thirstResearch into sports drinks and evaluation into which is best for athletes. (PAG B2, PAG B6, PAG B8)

Topic B4: Community level systems

Microorganisms play an important role in the continuous cycling of chemicals in ecosystems. Biotic and abiotic factors interact in an ecosystem and have an effect on communities. Living organisms form populations of single species, communities of many species and are part of ecosystems. Living organisms are interdependent and show adaptations to their environment. Feeding relationships reflect the stability of an ecosystem and indicate the flow of biomass through the ecosystem.

B4.1 Ecosystems

  • Summary: Microorganisms play an important role in the continuous cycling of chemicals in ecosystems. Biotic and abiotic factors interact in an ecosystem and have an effect on communities. Living organisms form populations of single species, communities of many species and are part of ecosystems.
  • Underlying knowledge and understanding: Learners should be familiar with the idea of a food web and the interrelationships associated with them and that variation allows living things to survive in the same ecosystem. They should also recognise that organisms affect their environment and are affected by it.
  • Common misconceptions: Research has shown that it is easier for a learner to explain the consequences on a food web if the producers are removed for some reason than if the top predators are taken away. It is also better to start off explaining ideas relating to food webs using small simple webs with animals and plants that learners are likely to know e.g. rabbits and foxes.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B4.1 Ecosystems - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM4.1icalculate rate changes in the decay of biological materialM1c
BM4.1iicalculate the percentage of massM1c
BM4.1iiiUse fractions and percentagesM1c
BM4.1ivplot and draw appropriate graphs selecting appropriate scales for the axesM4a and M4c
BM4.1vextract and interpret information from charts, graphs and tablesM2c and M4a

B4.1 Ecosystems - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B4.1a recall that many different materials cycle through the abiotic and biotic components of an ecosystemexamples of cycled materials e.g. nitrogen and carbon
B4.1b explain the role of microorganisms in the cycling of materials through an ecosystemthe role of microorganisms in decompositionResearch into the range of ecosystems and examples of micro-organisms that act as decomposers within them. (PAG B1, PAG B3, PAG B4, PAG B7)
B4.1c explain the importance of the carbon cycle and the water cycle to living organismsmaintaining habitats, fresh water, flow of nutrients and the stages of the carbon and water cycles
B4.1d explain the effect of factors such as temperature, water content, and oxygen availability on rate of decompositionthe terms aerobic and anaerobicM1c, M2c, M4a, M4cWS1.1b, WS1.1h, WS1.2b, WS1.2c, WS1.2e, WS1.3a, WS1.3b, WS1.3c, WS1.3d, WS1.3e, WS1.3f, WS1.3g, WS2a, WS2b, WS2c, WS2dInvestigation of the most favourable conditions for composting. (PAG B1, PAG B3, PAG B4, PAG B7)
B4.1e describe different levels of organisation in an ecosystem from individual organisms to the whole ecosystemM1c
B4.1f explain how abiotic and biotic factors can affect communitiestemperature, light intensity, moisture level, pH of soil, predators, foodM3a, M4a, M4cWS1.3a, WS1.3b, WS1.3e, WS1.3h, WS2a, WS2b, WS2c, WS2dIdentification of the biotic factors in an ecosystem using sampling techniques. (PAG B3)
B4.1g describe the importance of interdependence and competition in a communityinterdependence relating to predation, mutualism and parasitismWS1.4a, WS2a, WS2b, WS2c, WS2dExamination of the roots of a leguminous plant e.g. clover to observe the root nodules. (PAG B1)

Investigation of the holly leaf miner or the horse-chestnut leaf miner (Cameraria ohridella). (PAG B1, PAG B3)
B4.1h describe the differences between the trophic levels of organisms within an ecosystemuse of the terms producer and consumerInvestigation of the trophic levels within a children's story (e.g. The Gruffalo)
B4.1i describe pyramids of biomass and explain, with examples, how biomass is lost between the different trophic levelsloss of biomass related to egestion, excretion, respirationM1c, M4aWS1.3c, WS1.3eDiscussion of the best food source for humans (e.g. 'wheat vs. meat')

Production of ecological pyramids.
B4.1j calculate the efficiency of biomass transfers between trophic levels and explain how this affects the number of trophic levels in a food chainM1cCalculation of the biomass transfers using real data.

Topic B5: Genes, inheritance and selection

Topic B5 covers the genetic structures, mechanisms of inheritance, and evolutionary selection.

B5.1 Inheritance

  • Summary: Inheritance relies on the genetic information contained in the genome being passed from one generation to the next, whether sexually or asexually. The characteristics of a living organism are influenced by the genome and its interaction with the environment.
  • Underlying knowledge and understanding: Learners should be familiar with the idea of heredity as the process by which genetic information is passed from one generation to the next. They should have a simple model of chromosomes, genes and DNA.
  • Common misconceptions: Learners commonly struggle to appreciate the physical relationships between the nucleus, genetic material, the genome, chromosomes and genes. Accurate definitions of these terms will help learners' explanations in this topic. Learners often have well-developed (although not necessarily scientifically accurate) explanations for inheritance before undertaking GCSE study.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B5.1 Inheritance - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM5.1iunderstand and use direct proportions and simple ratios in genetic crossesM1c
BM5.1iiunderstand and use the concept of probability in predicting the outcome of genetic crossesM2e
BM5.1iiiextract and interpret information from charts, graphs and tablesM2c and M4a

B5.1 Inheritance - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B5.1a explain the following terms: gamete, chromosome, gene, allele/variant, dominant, recessive, homozygous, heterozygous, genotype and phenotypeUse of alleles to work out the phenotype of progeny.
B5.1b describe the genome as the entire genetic material of an organism
B5.1c describe that the genome, and its interaction with the environment, influence the development of the phenotype of an organismuse of examples of discontinuous (e.g. eye colour) and continuous variation (e.g. weight and height)
B5.1d Recall that all variants arise from mutations, and that most have no effect on the phenotype, some influence phenotype and a very few determine phenotype
B5.1e describe how genetic variants may influence phenotype:
•   in coding DNA by altering the activity of a protein
•   in non-coding DNA by altering how genes are expressed
•   in coding: DNA related to mutations affecting protein structure, including active sites of enzymes
•   in non-coding: DNA related to stopping transcription of mRNA (use of terms promoter, transcription factor not required)
B5.1f explain some of the advantages and disadvantages of asexual and sexual reproduction in a range of organismsthe number of live offspring per birth, how quickly the organisms can reproduce verses the need for the introduction of variation in a population caused by environmental pressures
B5.1g explain the terms haploid and diploid
B5.1h explain the role of meiotic cell division in halving the chromosome number to form gametesthat this maintains diploid cells when gametes combine and is a source of genetic variation
B5.1i explain single gene inheritancethe context of homozygous and heterozygous crosses involving dominant and recessive genesM2c, M4aPrediction of the probability of phenotype for genetic crosses.

Investigation into probability by suitable example (e.g. coin toss or die roll).
B5.1j predict the results of single gene crossesthe use of Punnett squaresM1c, M2c, M2e, M4a
B5.1k describe sex determination in humans using a genetic crossthe use of Punnett squaresM1c, M2c, M2e, M4a
B5.1l recall that most phenotypic features are the result of multiple genes rather than single gene inheritance
B5.1m describe the development of our understanding of geneticsthe work of MendelWS1.1a, WS1.1d, WS1.1f, WS1.1i

B5.2 Natural selection and evolution

Variation in the genome and changes in the environment drive the process of natural selection, leading to changes in the characteristics of populations. Evolution accounts for both biodiversity and how organisms are all related to varying degrees. Key individuals have played important roles in the development of our understanding of genetics.

B5.2 Context & Overview

  • Underlying knowledge and understanding: Learners should appreciate that changes in the environment can leave some individuals, or even some entire species, unable to compete and reproduce leading to extinction.
  • Common misconceptions: Learners are used to hearing the term evolution in everyday life but it is often used for items that have been designed and gradually improved in order to fit a purpose. They therefore find it difficult to grasp the idea that evolution by natural selection relies on random mutations. Learners also tend to imply that individuals change by natural selection.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B5.2 Natural selection and evolution - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B5.2a state that there is usually extensive genetic variation within a population of a species
B5.2b describe the impact of developments in biology on classification systemsnatural and artificial classification systems and use of molecular phylogenetics based on DNA sequencingWS1.1b
B5.2c explain how evolution occurs through the natural selection of variants that have given rise to phenotypes best suited to their environmentthe concept of mutation
B5.2d describe evolution as a change in the inherited characteristics of a population over time, through a process of natural selection, which may result in the formation of new species
B5.2e describe the evidence for evolutionfossils and antibiotic resistance in bacteriaWS1.1c, WS1.1d, WS1.1g
B5.2f describe the work of Darwin and Wallace in the development of the theory of evolution by natural selection and explain the impact of these ideas on modern biologyseedbanks being used as a store of biodiversityWS1.1a, WS1.1d, WS1.1g, WS1.1h, WS1.3i

Topic B6: Global challenges

This topic seeks to integrate learners' knowledge and understanding of biological systems and processes, with the aim of applying it to global challenges. Biological information is used to help people to improve their own lives and strive to create a sustainable world for future generations.

B6.1 Monitoring and maintaining the environment

  • Summary: Living organisms interact with each other, the environment and with humans in many different ways. If the variety of life is to be maintained we must actively manage our interactions with the environment. We must monitor our environment, collecting and interpreting information about the natural world, to identify patterns and relate possible cause and effect.
  • Underlying knowledge and understanding: From their study in topic B4, learners should be familiar with ecosystems and the various ways organisms interact. They should understand how biotic and abiotic factors influence communities. Learners should be familiar with the gases of the atmosphere from Key Stage 3.
  • Common misconceptions: It is important that in the study of this topic learners are given opportunities to explore both positive and negative human interactions within ecosystems.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B6.1 Monitoring the environment - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM6.1icalculate arithmetic meansM2b
BM6.1iiplot and draw appropriate graphs selecting appropriate scales for the axesM4a and M4c
BM6.1iiiunderstand and use percentilesM1c
BM6.1ivextract and interpret information from charts, graphs and tablesM2c and M4a
BM6.1vunderstand the principles of sampling as applied to scientific dataM2d

B6.1 Monitoring the environment - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B6.1a explain how to carry out a field investigation into the distribution and abundance of organisms in a habitat and how to determine their numbers in a given areasampling techniques (random and transects, capture-recapture), use of quadrats, pooters, nets, keys and scaling up methodsM2c, M2d, M3aWS1.2d, WS1.2b, WS1.2c, WS1.2e, WS1.3h, WS2a, WS2b, WS2c, WS2dInvestigation of ecological sampling methods. Using the symbols =, <, <<, >>, >, ∝, ~ in answers where appropriate. (PAG B1, PAG B3)

Investigation of sampling using a suitable model (e.g. measuring the red sweets in a mixed selection).
B6.1b describe both positive and negative human interactions within ecosystems and explain their impact on biodiversitythe conservation of individual species and selected habitats and threats from land use and huntingWS2a, WS2b, WS2c, WS2dInvestigation into the effectiveness of germination in different strengths of acid rain. (PAG B3, PAG B6)

Investigation into the effects of lichen distribution against pollution. (PAG B3)
B6.1c explain some of the benefits and challenges of maintaining local and global biodiversitythe difficulty in gaining agreements for and the monitoring of conservation schemes along with the benefits of ecotourism
B6.1d evaluate the evidence for the impact of environmental changes on the distribution of organisms, with reference to water and atmospheric gases

B6.2 Feeding the human race

The human population is increasing rapidly and with this comes a need for more food. Biologists are seeking to tackle this increased demand, which will lead to an improvement in the lives of many people around the world. However, there are many things to consider in achieving this aim, not least the impact on ecosystems.

B6.2 Context & Overview

  • Underlying knowledge and understanding: Learners should be familiar with the content of a healthy human diet and the consequences of imbalances in a healthy daily diet. Their knowledge and understanding from topics 1, 4 and 5 will also be drawn together in this topic. This includes the organisation of DNA, what plants require enabling them to photosynthesise, interactions between species and the idea of variability within species and subsequent selection of characteristics.
  • Common misconceptions: Learners can often think that genetic engineering leads to the increased use of pesticides.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B6.2 Feeding the human race - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM6.2iextract and interpret information from charts, graphs and tablesM2c and M4a

B6.2 Feeding the human race - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B6.2a describe some of the biological factors affecting levels of food securityincreasing human population, changing diets in wealthier populations, new pests and pathogens, environmental change, sustainability and cost of agricultural inputsM2b, M2f
B6.2b describe and explain some possible agricultural solutions to the demands of the growing human populationincreased use of hydroponics, biological control, gene technology, fertilisers and pesticidesWS1.1c
B6.2c explain the impact of the selective breeding of food plants and domesticated animalsM1c, M2c, M4aWS1.1cResearch into the Rothamsted Research Broadbalk experiment.
B6.2d describe genetic engineering as a process which involves modifying the genome of an organism to introduce desirable characteristics
B6.2e describe the main steps in the process of genetic engineeringrestriction enzymes, sticky ends, ligase, host bacteria and selection using antibiotic resistance markers, vectors e.g. plasmidsProduce a storyboard of the processes for genetic engineering.
B6.2f explain some of the possible benefits and risks of using gene technology in modern agriculturepractical and ethical considerationsWS1.1c, WS1.1d, WS1.1e, WS1.1f, WS1.1g, WS1.1h, WS1.3iResearch into the advantages and disadvantages of selective breeding and genetic engineering.
B6.2g explain some possible biotechnological solutions to the demands of the growing human populationgenetic modificationM1c, M2c, M4aWS1.1c, WS1.1gResearch into the growth of GM crops or livestock.

B6.3 Monitoring and maintaining health

Diseases affect the health of populations of both humans and plants. Scientists are constantly on the lookout for ways of preventing and combating disease. The prevention of disease in plants is important so that we are able to grow healthy plants enabling us to feed ourselves and enhance our environment.

B6.3 Context & Overview

  • Underlying knowledge and understanding: Learners should be familiar with the effects of 'recreational' drugs (including substance misuse) on behaviour, health and life processes, the impact of exercise, asthma and smoking on the gas exchange system and the consequences of imbalances in the diet, including obesity, starvation and deficiency diseases.
  • Common misconceptions: Research has shown that learners tend to view all micro-organisms as being non-beneficial. They tend to consider health as just physical and do not consider mental health. Learners also confuse which diseases are inherited and which are caught. They see cancer as a genetic disease.
  • Tiering: Statements shown in bold type will only be tested in the Higher Tier papers. All other statements will be assessed in both Foundation and Higher Tier papers.

B6.3 Maintaining health - Mathematical learning outcomes

ReferenceMathematical learning outcomesMathematical skills
BM6.3itranslate information between graphical and numerical formsM4a
BM6.3iiconstruct and interpret frequency tables and diagrams, bar charts and histogramsM2c
BM6.3iiiunderstand the principles of sampling as applied to scientific dataM2d
BM6.3ivuse a scatter diagram to identify a correlation between two variablesM2g
BM6.3vcalculate cross-sectional areas of bacterial cultures and clear agar jelly using πr²M5c

B6.3 Maintaining health - Topic content

Learning outcomesTo includeMathsWorking scientificallyPractical suggestions
B6.3a describe the relationship between health and disease
B6.3b describe different types of diseasescommunicable and non-communicable diseases
B6.3c describe the interactions between different types of diseaseHIV and tuberculosis; HPV and cervical cancerM4a
B6.3d explain how communicable diseases (caused by viruses, bacteria, protists and fungi) are spread in animals and plantsscientific quantities, number of pathogens, number of infected cases, estimating number of casesM2c, M2gWS1.4b
B6.3e explain how the spread of communicable diseases may be reduced or prevented in animals and plantsdetection of the antigen, DNA testing, visual identification of the diseaseM2cWS1.4b
B6.3f describe a minimum of one common human infection, one plant disease and sexually transmitted infections in humans including HIV/AIDShuman infections: one example of each viral, fungal, bacterial
plant diseases: viral tobacco mosaic virus TMV, fungal Erysiphe graminis barley powdery mildew, bacterial Agrobacterium tumefaciens crown gall disease
B6.3g describe physical plant defence responses to diseaseleaf cuticle, cell wall
B6.3h describe chemical plant defence responsesantimicrobial substances
B6.3i describe different ways plant diseases can be detected and identified, in the lab and in the fieldthe laboratory detection of the DNA or antigen from the disease causing organism. The field diagnosis by observation and microscopy
B6.3j explain how white blood cells and platelets are adapted to their defence functions in the blood
B6.3k describe the non-specific defence systems of the human body against pathogens
B6.3l explain the role of the immune system of the human body in defence against disease
B6.3m describe how monoclonal antibodies are producedWS1.1d
B6.3n describe some of the ways in which monoclonal antibodies can be usedtheir role in detecting antigens in pregnancy testing, detection of diseases (prostate cancer) and potentially treating disease (targeting cancer cells)
B6.3o explain the use of vaccines and medicines in the prevention and treatment of diseaseantibiotics, antivirals and antisepticsWS1.1g, WS1.1hResearch into whether children should be routinely vaccinated?
B6.3p explain the aseptic techniques used in culturing organismsuse of alcohol, flaming, autoclaving of glassware and growth media, and measures used to stop contaminants falling onto/into the growth media (e.g. working around a Bunsen burner)M3d, M5cWS1.1h, WS1.2c, WS2a, WS2b, WS2c, WS2dInvestigation into growth bacterial cultures using aseptic techniques. (PAG B1, PAG B7)
B6.3q describe the processes of discovery and development of potential new medicinespreclinical and clinical testingM2d, M3d, M5cWS1.1d, WS2a, WS2b, WS2c, WS2dInvestigation into growth bacterial cultures using aseptic techniques. (PAG B1, PAG B7)
B6.3r recall that many non-communicable human diseases are caused by the interaction of a number of factorscardiovascular diseases, many forms of cancer, some lung (bronchitis) and liver (cirrhosis) diseases and diseases influenced by nutrition, including type 2 diabetes
B6.3s evaluate some different treatments for cardiovascular diseaselifestyle, medical and surgicalM2g, M2d
B6.3t analyse the effect of lifestyle factors on the incidence of non-communicable diseases at local, national and global levelslifestyle factors to include exercise, diet, alcohol and smokingM2c, M2d, M4a
B6.3u describe cancer as the result of changes in cells that lead to uncontrolled growth and division
B6.3v discuss potential benefits and risks associated with the use of stem cells in medicinetissue transplantation and rejectionWS1.1c, WS1.1d, WS1.1e, WS1.1f, WS1.1g, WS1.1h, WS1.j
B6.3w explain some of the possible benefits and risks of using gene technology in medicinepractical and ethical considerationsWS1.1c, WS1.1d, WS1.1e, WS1.j
B6.3x discuss the potential importance for medicine of our increasing understanding of the human genomethe ideas of predicting the likelihood of diseases occurring and their treatment by drugs which are targeted to genomesWS1.1c, WS1.1d, WS1.j

Topic B7: Practical skills

To view the practical skills section, please go to the dedicated practical skills section.